HIF-1α silencing downregulates SLC37A2, SLC37A3, and G6PC3 gene expression and impacts glioblastoma stemness features in 3D neurospheres

Our findings suggest that GBM-derived 3D neurosphere cultures exhibit HIF-1α-regulated molecular features consistent with CSC and a potential mimicry of chemoresistance-associated traits rather than a direct demonstration of chemoresistance. These correlative observations support a potential link between metabolic reprogramming and the emergence of such phenotypes in response to hypoxic stress. Further investigation is needed to establish causal relationships and to better understand the underlying mechanisms.