Electrochemically-generated ferricyanide enables thiol–ene capture of protein–protein binding

Due to its efficiency, selectivity and biocompatibility, the thiol-ene coupling has demonstrated its potential for protein labelling. Here, we report the electrochemical thiol-ene activation to capture protein-protein binding events. <i>In situ</i> electrochemical generation of Fe(III) was used as the first example of an electrochemically generated thiol-ene protein-protein labelling reaction.