The Diagnostic Value of Autofluorescence in Hematoxylin–Eosin‐Stained Sections for Cutaneous Sporotrichosis

Autofluorescence enables rapid Sporothrix detection with perfect specificity and excellent interobserver agreement, but its low sensitivity limits standalone use. PAS and GMS remain superior for definitive diagnosis. While its reagent-free, cost-effective nature supports adjunct screening where special stains are unavailable, erythrocyte interference and technical variability require protocol optimization.