Effect of intercropping on soil microbial diversity and community network

The results showed that the intercropping and sampling location markedly affected bacterial a-diversity, with SW showing a 68.7% higher Shannon index and a 15.0% higher Simpson index than W. Although there were no significant differences in eukaryotic α-diversity and β-diversity between SW and W treatments, unique species distributions were observed. Co-occurrence network analysis revealed that intercropping enhanced the complexity and stability of both the bacterial and eukaryotic communities. Distance-based redundancy analysis (dbRDA) indicated that the soil properties, particularly total phosphorus, available phosphorus, pH, and easily oxidizable carbon, were significantly correlated with the bacterial community composition. While easily oxidizable carbon was the main factor influencing soil eukaryotic community. In conclusion, SW positively regulates soil microbial communities, enhancing bacterial diversity and fostering more stable microbial networks. This study provides a theoretical basis for adopting intercropping to promote agricultural sustainability. Nonetheless, long-term research is needed to explore community shift functions and their long-term impacts on soil health and productivity for sustainable farming.