Optimised dissociation and multimodal profiling of prostate cancer stroma reveal fibromuscular cell heterogeneity with clinical correlates
This study presents a robust workflow for improved isolation and characterisation of fibromuscular stromal cells in PCa. The multimodal approach enabled refined characterisation of phenotypically distinct and clinically-relevant stromal subpopulations within their spatial context providing a foundation for future TME-targeted therapies.
Authors
- Lucy Neumann
- Elisabeth Damisch (ORCID: https://orcid.org/0000-0003-0833-8412)
- Marianna Kruithof‐de Julio (ORCID: https://orcid.org/0000-0002-6085-7706)
- Georg Schäfer (ORCID: https://orcid.org/0009-0004-7622-7401)
- Sieghart Sopper (ORCID: https://orcid.org/0000-0003-2265-1974)
- Georgios Fotakis (ORCID: https://orcid.org/0000-0001-9659-4226)
- Martin Puhr (ORCID: https://orcid.org/0000-0002-3858-9653)
- Zlatko Trajanoski (ORCID: https://orcid.org/0000-0002-0636-7351)
- Natalie Sampson (ORCID: https://orcid.org/0000-0001-9326-8177)
- Lukas Nommensen (ORCID: https://orcid.org/0009-0007-4549-9430)
- Isabel Heidegger
- Edward Brunner (ORCID: https://orcid.org/0009-0000-2836-6889)
Institutions
- University of Bern (CH)
- Innsbruck Medical University (AT)
- Universität Innsbruck (AT)
- University Hospital of Bern (CH)
- Tyrolean Cancer Research Institute (AT)
Publication Details
- Journal
- Frontiers in Cell and Developmental Biology
- Published
- 2025-11-25
- DOI
- https://doi.org/10.3389/fcell.2025.1653780
- Primary Topic
- Cancer Cells and Metastasis
- Type
- article
- Field-Weighted Citation Impact
- 0.00