Evaluation of the impact of vitamin D supplementation on the coagulation profile in mice

Abstract Background Vitamin D is an essential molecule involved in several physiological processes and has demonstrated considerable benefit as a medicinal agent. Thus, we aimed to examine the effects of vitamin D supplementation on hematological, coagulation parameters, and liver markers. Methods Thirty mice were evenly allocated into three groups: the control group, the vitamin D-treated group, and the heparin-treated group. Following a four-month period, body weight, hematological and coagulation parameters, along with liver enzymes, were assessed and examined utilizing one-way ANOVA. Results Data showed that the mice treated with vitamin D had higher body weights than those in the control group. The data also revealed no significant differences in complete blood count parameters between the mice administered with vitamin D, the controls and the heparin group. However, the levels of prothrombin time (PT) and activated partial thromboplastin time (aPTT) were markedly increased in the vitamin D–treated group relative to the controls and the heparin group. In addition, no significant differences were observed in fibrinogen levels between the three groups. The data also showed that vitamin D supplementation had no major impact on alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) levels with a normal liver architecture. Conclusions Vitamin D supplementation resulted in prolonged PT and aPTT levels. These findings indicate that vitamin D potentially possesses antithrombotic properties and may be used in thrombotic episodes. A comprehensive assessment of vitamin D impact on the hemostatic system using supplementary indicators for coagulation and thrombosis is necessary.