Molecular characterisation of human penile carcinoma and generation of paired epithelial primary cell lines
Penile carcinoma is a rare malignancy in developed countries but is more common in South America and East Africa. The small number of cases means there are limited resources to investigate disease pathogenesis. This report describes a method of generating primary cell lines from freshly isolated human penile tissue using a clonal expansion approach on mitotically inactivated fibroblasts. Matched normal and penile cancer cell lines from two patients were generated and characterised. Molecular karyotyping and targeted sequencing were performed to compare their genomic landscape. Gains in 8q13.3, 10q23.2, 10q25.1, 10q26.13,12p13.33, 20q13.33, Xq21.1 or losses in Yq11.23 were consistent in both tumour cell lines. Gains in 8q13.3 and Xq21.1 cytobands positively correlated with changes in the expression of nearby genes. The top 20 differentially expressed genes are involved in immune responses like interferon alpha/beta signalling. Additionally, there was an increase in integrin β1, transglutaminase 1, keratins 5, 10, 14 and 16, and a decrease in involucrin protein expression. The cell lines described in this study can provide an invaluable platform for new insights and testing of therapies for penile carcinoma.
Authors
- Karolina Penderecka
- Matteo Vietri Rudan (ORCID: https://orcid.org/0000-0002-1798-8241)
- Simon Broad
- Saif Khan (ORCID: https://orcid.org/0000-0002-4756-217X)
- Rifat Hamoudi (ORCID: https://orcid.org/0000-0002-1402-0868)
- Kalle Sipilä (ORCID: https://orcid.org/0000-0002-8882-3786)
- Rui Henrique (ORCID: https://orcid.org/0000-0003-3171-4666)
- Aamir Ahmed (ORCID: https://orcid.org/0000-0001-7405-5336)
- Manit Arya (ORCID: https://orcid.org/0000-0002-5122-6881)
- Tom Bertin (ORCID: https://orcid.org/0000-0001-5411-9531)
- Asıf Muneer (ORCID: https://orcid.org/0000-0003-2958-1614)
- Mahmood Yaseen Hachim (ORCID: https://orcid.org/0000-0002-7096-0731)
- Natalie Bergmoser
Institutions
- Universidade do Porto (PT)
- King's College London (GB)
- Princess Alexandra Hospital NHS Trust (GB)
- University of Sharjah (AE)
- National Institute for Health and Care Research (GB)
- Institute for Stem Cell Biology and Regenerative Medicine (IN)
- MRC Laboratory for Molecular Cell Biology (GB)
- Instituto Português de Oncologia Francisco Gentil (PT)
- Mohammed Bin Rashid University of Medicine and Health Sciences (AE)
- MRC Centre for Regenerative Medicine (GB)
- Wellcome / EPSRC Centre for Interventional and Surgical Sciences (GB)
- Princess Alexandra Hospital (GB)
- Institute of Biomedical Science (GB)
- University College London (GB)
Publication Details
- Journal
- Molecular Oncology
- Published
- 2025-11-25
- DOI
- https://doi.org/10.1002/1878-0261.70156
- Primary Topic
- Genital Health and Disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institute for Health and Care Research
- King's College London
- University College London