Interaction Between Enhancers and Promoters in Chicken Genome

Gene expression from promoters is influenced by interactions with genomic enhancers located within the same topologically associating domain (TAD). Enhancer activity can be evaluated by measuring the transcriptional output of enhancer RNAs, and the CAGE methodology enables the simultaneous assessment of enhancer and promoter activities within a single experiment. In this study, we examined the correlation between gene and enhancer activities within individual TADs across multiple tissues in slow- and fast-growing chickens, and we assessed the biological significance of genes with promoters that are regulated by enhancers. Our analysis revealed a statistically significant association between gene expression levels and enhancer activity in all tissues examined. Notably, enhancer-mediated regulation appears to activate key pathways involved in transcriptional control and nucleic acid biosynthesis.