Plateau-sourced Morchella sextelata lipids: Distinct composition and antithrombotic properties

Morels ( Morchella spp.) are a highly valued edible and medicinal fungus. In this study, M. sextelata sourced from plateau regions was analyzed for its lipid composition and antithrombotic activities. Ultraperformance liquid chromatography coupled with mass spectrometry (UPLC-MS) analysis of mature fruiting bodies identified 203 lipid molecules, grouped into 4 categories and 14 subclasses. Glycerolipids predominated (59.98% of total lipids), with triacylglycerols as the most abundant subclass (38.78%). Anti-platelet aggregation assays revealed that semi-polar and highly polar lipids showed comparable activity to ginkgolide B (positive control) in the thrombin - induced model, with IC 50 values of 314.20 ± 19.86 ng/mL and 327.13 ± 12.61 ng/mL, respectively. This study enhances our understanding of habitat-specific lipid profiles in M. sextelata and supports its potential as a source of functional lipids. • 203 lipid molecules were identified from plateaus-sourced Morchella sextelata. • Habitat divergence significantly reshapes lipid profiles in M. sextelata. • Reduced free fatty acids in plateau- vs. plain-sourced M. sextelata. • AE/ME fractions match ginkgolide B (positive control) in thrombin inhibition.