Rumen Fluid Metabolomics and Microbiome Profiling of Dairy Cows Fed Combinations of Prebiotics, Essential Oil Blend, and Onion Peel Using the RUSITEC System
These results demonstrate that dietary supplementation with GEO, MEO, or OLEO modulates rumen metabolism and microbial ecology without impairing fermentation, supporting improved nutrient utilization, antioxidant defenses, and metabolic resilience in dairy cows, with potential benefits for productivity and sustainability.
Authors
- Chika C. Anotaenwere (ORCID: https://orcid.org/0009-0004-2673-4130)
- O. O. Adelusi (ORCID: https://orcid.org/0000-0002-8723-9190)
- O. A. Oderinwale (ORCID: https://orcid.org/0000-0001-6220-9158)
- Kelechi A. Ike (ORCID: https://orcid.org/0009-0009-8169-7404)
- Ahmed Eid KHOLIF (ORCID: https://orcid.org/0000-0003-0472-4770)
- J.O. Alabi (ORCID: https://orcid.org/0000-0003-1442-5213)
- Uchenna Y. Anele (ORCID: https://orcid.org/0000-0002-6630-9292)
- Michael Wuaku (ORCID: https://orcid.org/0009-0001-7584-8888)
- Deborah O. Okedoyin (ORCID: https://orcid.org/0009-0001-9779-8738)
- John Odunayo Adebayo
- James M. Enikuomehin (ORCID: https://orcid.org/0000-0003-3194-5974)
- Andrea R. Gentry-Apple
Institutions
- North Carolina Agricultural and Technical State University (US)
- National Research Centre (EG)
Publication Details
- Journal
- Metabolites
- Published
- 2025-11-25
- DOI
- https://doi.org/10.3390/metabo15120762
- Primary Topic
- Ruminant Nutrition and Digestive Physiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Agriculture
- North Carolina Agricultural and Technical State University
- National Institute of Food and Agriculture