Detection of intestinal parasites in leopard cat fecal samples using shotgun metagenomics

The leopard cat (Prionailurus bengalensis) is a wild felid species that serves as a reservoir of zoonotic parasites. In this study, we investigated intestinal parasite taxa by reanalyzing previously published shotgun metagenomic sequencing data from fecal samples of wild leopard cats using a custom 18S rRNA gene reference database constructed from the NCBI nucleotide database. Among 11 metagenomic samples, 5 parasite species were identified: Toxoplasma gondii, Clonorchis sinensis, Strongyloides planiceps, Cylicospirura petrowi, and Pharyngostomum cordatum. These findings demonstrate that shotgun metagenomic analysis of fecal samples can be a useful tool for monitoring zoonotic parasite infections in this species and for investigating parasite life cycles. However, this approach is limited by its dependence on existing reference databases and requires experimental validation of the findings.

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Publication Details

Journal
Parasites Hosts and Diseases
Published
2025-11-19
DOI
https://doi.org/10.3347/phd.25032
Primary Topic
Toxoplasma gondii Research Studies
Type
article
Field-Weighted Citation Impact
0.00

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article

Detection of intestinal parasites in leopard cat fecal samples using shotgun metagenomics

Xavier Chavarría, Du‐Yeol Choi, Ju Yeong Kim, Myung‐hee Yi et al.
Parasites Hosts and Diseases
Toxoplasma gondii Research Studies
article

Detection of intestinal parasites in leopard cat fecal samples using shotgun metagenomics

Xavier Chavarría, Du‐Yeol Choi, Ju Yeong Kim, Myung‐hee Yi, Seongjun Choe, Singeun Oh, Seung‐Hun Lee, Jun Ho Choi, Yoon Hee Cho, Dongjun Kang, Arwa Shatta
article en

Abstract

The leopard cat (Prionailurus bengalensis) is a wild felid species that serves as a reservoir of zoonotic parasites. In this study, we investigated intestinal parasite taxa by reanalyzing previously published shotgun metagenomic sequencing data from fecal samples of wild leopard cats using a custom 18S rRNA gene reference database constructed from the NCBI nucleotide database. Among 11 metagenomic samples, 5 parasite species were identified: Toxoplasma gondii, Clonorchis sinensis, Strongyloides planiceps, Cylicospirura petrowi, and Pharyngostomum cordatum. These findings demonstrate that shotgun metagenomic analysis of fecal samples can be a useful tool for monitoring zoonotic parasite infections in this species and for investigating parasite life cycles. However, this approach is limited by its dependence on existing reference databases and requires experimental validation of the findings.

Parasites Hosts and DiseasesVol. 63(4)
Chungbuk National University (KR), Instituto de Medicina Tropical (PY), Chungbuk National University Hospital (KR)
National Research Foundation, Korea Health Industry Development Institute, National Research Foundation of Korea, Ministry of Science and ICT, South Korea
Openalex Percentile: Top 62%
Toxoplasma gondii Research Studies
DOIOpen Access PDFOpenAlex
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Detection of intestinal parasites in leopard cat fecal samples using shotgun metagenomics — Xavier Chavarría, Du‐Yeol Choi, et al. · Parasites Hosts and Diseases (2025) | TGRS Research Map | TGRS