Interleukin-6 accelerates the progression of warm autoimmune hemolytic anemia by affecting the DNA methylation status of peripheral blood B lymphocytes
DNA methylation of peripheral blood (PB) B cells of warm autoimmune hemolytic anemia (wAIHA) patient was investigated. B cell DNA methylation level and the methylation-regulatory genes mRNA from 34 wAIHA patients and 16 healthy controls (HCs) were detected. The correlation between B cell DNA methylation and clinical indicators was analyzed. The effects of IL-6 on the B cell DNA methylation were examined. B cell DNA methylation level and DNMT1 mRNA expression of hemolytic episode patients were lower than that in remission patients and HCs. B cell DNA methylation in glucocorticoid-resistant patients was low. The hemolytic degree, response and relapse rate were correlated with B cell DNA methylation. Serum IL-6 concentration in hemolytic episode patients was higher than that in HCs and IL-6 inhibited DNMT1 expression in B cell of patients. IL-6 may inhibit the B cell DNA methylation levels and methylation regulatory genes of wAIHA patients.
Authors
- Manjun Zhao (ORCID: https://orcid.org/0000-0003-1430-9570)
- Limin Xing (ORCID: https://orcid.org/0000-0002-0182-8892)
- Huaquan Wang (ORCID: https://orcid.org/0000-0001-8136-4196)
- Zhaoyun Liu (ORCID: https://orcid.org/0000-0002-2730-1562)
- Rong Fu (ORCID: https://orcid.org/0000-0002-2850-1697)
- Zonghong Shao
- Yang Zhang (ORCID: https://orcid.org/0000-0002-3476-8937)
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Peking Union Medical College Hospital (CN)
- Tianjin Medical University General Hospital (CN)
Publication Details
- Journal
- Scientific Reports
- Published
- 2025-11-25
- DOI
- https://doi.org/10.1038/s41598-025-29813-6
- Primary Topic
- Blood groups and transfusion
- Type
- article
- Field-Weighted Citation Impact
- 0.00