Corrigendum to “A renal biopsy-anchored multi-marker signature involving AOPEP SNP-driven splicing, miR-27b-3p and glycated albumin for stratifying renal damage in type 2 diabetes”. [DIAB 229 (2025) 112460]
Aims: Stratifying renal damage in type 2 diabetes is challenging due to overlapping features between diabetic nephropathy (DN) and non-diabetic renal disease (NDRD). While miR-27b-3p modulates kidney fibrosis in DN through the lysine63 ubiquitination pathway, its upstream regulation and diagnostic relevance remain unclear. Methods: In a biopsy-verified cohort of 231 chronic kidney disease (CKD) patients with and without type 2 diabetes, we investigated whether AOPEP promoter SNPs drive alternative splicing affecting intronic miR-27b-3p expression. We also assessed the diagnostic utility of combining these biomarkers with clinical parameters, such as glycated albumin (GA), to distinguish DN from NDRD. Results: The rs10761364 minor allele was associated with reduced urinary miR-27b-3p and AOPEP splicing that excluded exons hosting the miR-23b/27b/24 cluster. This pattern, observed in vivo and under hyperglycemia in vitro, led to elevated AOPEP protein isoforms. A model combining rs10761364, GA, and urinary miR-27b-3p showed high diagnostic accuracy (AUC up to 0.93) in discriminating DN from NDRD. miR-27b-3p inversely correlated with GA, and GA-based models outperformed those using HbA1c. Conclusion: We identify a genotype- and glucose-dependent mechanism regulating miR-27b-3p via AOPEP splicing and propose a biopsy-anchored, non-invasive biomarker panel (rs10761364, GA, miR-27b-3p) to differentiate DN from NDRD, supporting personalized nephrology care.
Authors
- Fabio Sallustio
- Claudia Cinefra
- Annalisa Schirinzi (ORCID: https://orcid.org/0000-0001-9826-7907)
- Adriano Montinaro
- Viji Nair (ORCID: https://orcid.org/0000-0003-1657-954X)
- Maria Felicia Faienza (ORCID: https://orcid.org/0000-0002-1899-8337)
- Giovanni Stallone (ORCID: https://orcid.org/0000-0002-2862-9017)
- Luigi Laviola (ORCID: https://orcid.org/0000-0001-8860-5845)
- Michele Rossini (ORCID: https://orcid.org/0000-0002-5801-4136)
- Elena Squiccimarro (ORCID: https://orcid.org/0000-0002-6419-8488)
- Anna Gallone (ORCID: https://orcid.org/0000-0002-2252-9584)
- Paola Pontrelli (ORCID: https://orcid.org/0000-0002-7654-8318)
- Maria F. Gomez (ORCID: https://orcid.org/0000-0001-6210-3142)
- Francesca Conserva (ORCID: https://orcid.org/0000-0003-2863-864X)
- Francesco Pesce (ORCID: https://orcid.org/0000-0002-2882-4226)
- Ighli di Bari
- Edgar A. Otto (ORCID: https://orcid.org/0000-0002-2387-9973)
- Francesco Giorgino (ORCID: https://orcid.org/0000-0001-7372-2678)
- Gianluigi Zaza (ORCID: https://orcid.org/0000-0002-6004-6196)
- Matthias Kretzler (ORCID: https://orcid.org/0000-0003-4064-0582)
- Francesca Di Serio (ORCID: https://orcid.org/0000-0001-6089-1165)
- Marco Fiorentino (ORCID: https://orcid.org/0000-0001-7334-8372)
- Tommaso Maria Marvulli (ORCID: https://orcid.org/0000-0001-9962-1955)
- Alessandra Stasi (ORCID: https://orcid.org/0000-0003-3652-3623)
- Giorgia Sclavo (ORCID: https://orcid.org/0009-0007-4390-7015)
- Damian Fermin (ORCID: https://orcid.org/0000-0001-9678-6834)
- Rajasree Menon
- Loreto Gesualdo
Institutions
- University of Foggia (IT)
- Lund University (SE)
- University of Michigan (US)
- Agostino Gemelli University Polyclinic (IT)
- Azienda Universitaria Ospedaliera Consorziale - Policlinico Bari (IT)
- Michigan Medicine (US)
- University of Calabria (IT)
- University of Bari Aldo Moro (IT)
Publication Details
- Journal
- Diabetes Research and Clinical Practice
- Published
- 2025-11-25
- DOI
- https://doi.org/10.1016/j.diabres.2025.113009
- Primary Topic
- Chronic Kidney Disease and Diabetes
- Type
- article
- Field-Weighted Citation Impact
- 0.00