Immobilization-free SELEX for aptamer discovery targeting colorectal cancer-derived small extracellular vesicles
Our findings demonstrate the applicability of SELEX technology to native sEVs and highlight the diagnostic potential of the identified aptamers for sEV-based cancer detection. The EDGE-SELEX method and the G6 motif may serve as valuable tools for future clinical applications in non-invasive cancer diagnostics and aptamer engineering, although further validation with clinical samples is warranted.
Authors
- Jinseo Son
- Byung Seok
- Seung Hyeon Reo (ORCID: https://orcid.org/0000-0003-3085-3250)
- Junhyeong Kim (ORCID: https://orcid.org/0000-0001-7747-5475)
- Ki Soo Park (ORCID: https://orcid.org/0000-0002-0545-0970)
- Eun Sung Lee
Institutions
- Konkuk University (KR)
Publication Details
- Journal
- Journal of Nanobiotechnology
- Published
- 2025-11-25
- DOI
- https://doi.org/10.1186/s12951-025-03813-0
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- National Research Foundation of Korea
- Ministry of Science and ICT, South Korea