Functional characterization of double-negative T cells isolated from leukoreduction filter residues
DNT cells derived from leukoreduction filter residues exhibited highly comparable characteristics to those from whole blood in terms of expansion, purity, differentiation, and biological potency. Furthermore, their biological activity post-cryopreservation and revival remained largely similar to non-cryopreserved cells. These findings suggest that leukoreduction filter residues represent a promising alternative source of starting material for manufacturing off-the-shelf, allogeneic DNT cell therapeutics.
Authors
- Longlong Sang (ORCID: https://orcid.org/0000-0002-0521-1478)
- Frances Wu (ORCID: https://orcid.org/0000-0002-3644-6026)
- Liming Yang (ORCID: https://orcid.org/0000-0002-0390-8845)
- Yue Wu (ORCID: https://orcid.org/0000-0002-4102-4055)
- Kaiyu Huang
- Zhiqiang Xiang
Institutions
- Zhejiang Chinese Medical University (CN)
- Shaoxing University (CN)
- Shaoxing City Women and Children Hospital (CN)
- Shaoxing People's Hospital (CN)
- Zhejiang Medicine (China) (CN)
Publication Details
- Journal
- Journal of Zhejiang University (Medical Sciences)
- Published
- 2025-11-20
- DOI
- https://doi.org/10.3724/zdxbyxb-2025-0625
- Primary Topic
- CAR-T cell therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00