Generation of hiPSC-Derived Brain Microvascular Endothelial Cells Using Directed Differentiation and Transcriptional Reprogramming
This protocol generates rBMECs with strong BBB properties and a brain-specific transcriptome signature. In addition, the iPSC-derived 3D neurovascular unit system shows some similar properties to the in vivo human BBB. Finally, familial Alzheimer disease mutation <i>APP V717I</i> alters several BBB-related properties of rBMECs and their inflammatory state, independent of Aβ42 (amyloid-β [1-42] peptide).
Authors
- Andrew A. Sproul (ORCID: https://orcid.org/0000-0001-6972-1592)
- Emily Richters (ORCID: https://orcid.org/0000-0002-8964-726X)
- Uğur Akcan (ORCID: https://orcid.org/0000-0002-6903-7712)
- Dritan Agalliu (ORCID: https://orcid.org/0000-0002-5375-4143)
- Paula Barrilero Delgado
- Ronak Patel (ORCID: https://orcid.org/0000-0001-6782-6470)
- Aomeng Cui (ORCID: https://orcid.org/0000-0002-0505-1275)
- Patrick Bosco
Institutions
- Alzheimer's Association (US)
- Universidad Francisco de Vitoria (ES)
- Columbia University Irving Medical Center (US)
- Institute of Molecular and Cell Biology (RU)
- Anna Needs Neuroblastoma Answers (US)
Publication Details
- Journal
- Arteriosclerosis Thrombosis and Vascular Biology
- Published
- 2025-11-25
- DOI
- https://doi.org/10.1161/atvbaha.125.323397
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00