JBIR-160–166 Obtained from the Heterologous Expression of a Cryptic Gene Cluster in <i>Kitasatospora setae</i>
The heterologous expression of unidentified gene clusters is a strategy for obtaining new natural products. Acyltransferases in polyketide synthases (PKSs) are responsible for loading extender units. Notably, the type I PKS biosynthetic gene cluster <i>kse</i> obtained from <i>Kitasatospora setae</i> Δ<i>ksbA</i> features the alkylmalonyl-CoA-accepting acyltransferase domain. The heterologous expression of <i>kse</i> clusters produces new compounds designated as JBIR-160-166. Herein, we report the heterologous expression, isolation, and structure determination of these compounds.
Authors
- Junko Hashimoto
- Ikuko Kozone (ORCID: https://orcid.org/0000-0001-6298-1121)
- Shunji Takahashi (ORCID: https://orcid.org/0000-0003-1684-9298)
- Noritaka Kagaya (ORCID: https://orcid.org/0000-0002-0622-983X)
- Kazuo Shin‐ya (ORCID: https://orcid.org/0000-0002-4702-0661)
- Yusuke Ogura (ORCID: https://orcid.org/0000-0002-8646-9322)
- Keita Amagai (ORCID: https://orcid.org/0000-0001-5804-4047)
- Kei Kudo (ORCID: https://orcid.org/0000-0001-7500-9462)
- Haruo Ikeda
- Hirosato Takikawa (ORCID: https://orcid.org/0000-0002-3668-8834)
- Hikaru Suenaga (ORCID: https://orcid.org/0000-0003-2218-9273)
- Takehiro Nishimura (ORCID: https://orcid.org/0000-0002-6600-9193)
- Miho Izumikawa (ORCID: https://orcid.org/0000-0001-5277-5260)
Institutions
- Keio University (JP)
- Japan Biological Informatics Consortium (JP)
- RIKEN Advanced Science Institute (JP)
- Kitasato University (JP)
- National Institute of Advanced Industrial Science and Technology (JP)
- The University of Tokyo (JP)
Publication Details
- Journal
- Organic Letters
- Published
- 2025-11-24
- DOI
- https://doi.org/10.1021/acs.orglett.5c03682
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00