A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L
This system provides a reliable protocol for isolation, transfection and culture of cannabis protoplasts. It also offers a framework for investigating gene function, as well as advancing protoplast fusion and genome editing technologies for this species.
Authors
- Kamil Szymonik (ORCID: https://orcid.org/0000-0002-0821-2396)
- Katarzyna Stelmach (ORCID: https://orcid.org/0000-0002-9435-8753)
- Andrew Maxwell Phineas Jones (ORCID: https://orcid.org/0000-0001-9106-5341)
- Ewa Grzebelus (ORCID: https://orcid.org/0000-0003-2067-585X)
Institutions
- University of Agriculture in Krakow (PL)
- University of Guelph (CA)
Publication Details
- Journal
- BMC Plant Biology
- Published
- 2025-11-24
- DOI
- https://doi.org/10.1186/s12870-025-07686-1
- Primary Topic
- Cannabis and Cannabinoid Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00