Differential effects of plant growth regulators and carbohydrates on in vitro propagation of Scutellaria barbata D. Don

Experiments were conducted to establish the procedure for sterilizing single-node explants from the mother plant of barbed skullcap Scutellaria barbata L7 line (characterized by high scutellarin content) grown in the greenhouse and to induce organogenesis. The effect of different PGRs and carbohydrates on shoot number and shoot length was investigated. The largest number of shoots per explant (16.4) was formed after treatment with 3 mg·dm−3 BAP. Shoot multiplication occurred most intensively on medium with the combination of 0.09 M sucrose and 1 mg·dm−3 KIN (9.8 shoots per explant), and their elongation on the medium with 1 mg·dm−3 GA3 (10.0 cm). The rhizogenesis process was intensified by using 2 mg·dm−3 IBA (87%). Regenerated, rooted plants were acclimatized to ex vitro conditions, planted in pots, and placed in a greenhouse.