Update and New Implementation of the MIRAGE Reporting Guidelines for Mass Spectrometry Experiments in Glycoscience
The MIRAGE (Minimum Information Required for A Glycomics Experiment) guidelines for mass spectrometry (MS) data were initially developed to standardize the reporting of instrumentation, data acquisition and analytical details of the MS-based identification of released glycans. However, the growing interest in the study of intact glycoproteins and recent advances in MS-based glycoproteomics now necessitate a revision and expansion of these guidelines. This update includes an enhanced section focused on glycan structure analysis (glycomics) and introduces a new component tailored to the specific requirements of glycoproteomics. It addresses both shared and unique aspects of each approach and highlights glycoinformatics resources designed to facilitate data submission in compliance with the updated standards.
Authors
- Carsten Kettner (ORCID: https://orcid.org/0000-0002-8697-6842)
- Weston B. Struwe (ORCID: https://orcid.org/0000-0003-0594-226X)
- Michael Tiemeyer (ORCID: https://orcid.org/0000-0002-8704-9143)
- Daniel Kolarich (ORCID: https://orcid.org/0000-0002-8452-1350)
- Niclas G. Karlsson (ORCID: https://orcid.org/0000-0002-3045-2628)
- Joshua Klein (ORCID: https://orcid.org/0000-0003-1279-6838)
- Frédérique Lisacek (ORCID: https://orcid.org/0000-0002-0948-4537)
- William E. Hackett (ORCID: https://orcid.org/0000-0001-8225-0916)
- Kiyoko F. Aoki‐Kinoshita (ORCID: https://orcid.org/0000-0002-6662-8015)
- Nicolle H. Packer (ORCID: https://orcid.org/0000-0002-7532-4021)
- Joseph Zaia (ORCID: https://orcid.org/0000-0001-9497-8701)
- Yushi Takahashi (ORCID: https://orcid.org/0000-0002-9210-6413)
- Shujiro Okuda (ORCID: https://orcid.org/0000-0002-7704-8104)
- Morten Thaysen‐Andersen (ORCID: https://orcid.org/0000-0001-8327-6843)
- Marissa L Maciej-Hulme
Institutions
- Boston University (US)
- Griffith University (AU)
- SIB Swiss Institute of Bioinformatics (CH)
- University of Georgia (US)
- OsloMet – Oslo Metropolitan University (NO)
- Beilstein-Institut (DE)
- University of Oxford (GB)
- Soka University (JP)
- ARC Centre of Excellence in Synthetic Biology (AU)
- Carbohydrate Competence Center (NL)
- Niigata University (JP)
Publication Details
- Journal
- Molecular & Cellular Proteomics
- Published
- 2025-11-24
- DOI
- https://doi.org/10.1016/j.mcpro.2025.101473
- Citations
- 1
- Primary Topic
- Glycosylation and Glycoproteins Research
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Beilstein-Institut zur Förderung der Chemischen Wissenschaften