Ready-to-use hemostatic moldable bioactive bone putty for bone trauma treatment

For a long time, the clinical management of traumatic bone injuries has been limited by the constraints of bone wax as a hemostatic material. To tackle these clinical challenges, this study has developed a ready-to-use hemostatic moldable bioactive bone putty (BP) that excels in rapid hemostasis and promotes bone regeneration. BP is a formulation made from polyethylene glycol and pre-gelatinized hydroxypropyl distarch phosphate as a molding agent, along with calcium sulfate bone cement and calcium phosphate bone cement as bioactive components. While the application method remains the same as that of bone wax, BP encourages swift thrombus formation, significantly reducing coagulation time and blood loss. Additionally, BP improves its mechanical strength through hydration reactions upon exposure to bodily fluids, providing essential support for bone tissues while completely degrading within 8 weeks of implantation. In vitro cell studies have underscored the excellent biocompatibility of BP, demonstrating its capacity to stimulate the proliferation of BMSCs, increase the expression of key osteogenic genes, and increase calcium nodule deposition. Histological analyses have further revealed the positive impact of BP on osteogenesis, whereas in vivo experiments have shown significant inhibition of inflammatory fiber formation. Overall, the BP has emerged as an innovative solution for accelerating hemostasis and facilitating regenerative repair in cases of bone injury.