Synthesis, Characterization, and Study of Biological, Laser, and Molecular Docking Activity of Oxazolidinone‐5‐one Compounds Derived From 5‐methyl 1,3,4‐thiadiazole‐2‐amine

ABSTRACT In this study, 5‐methyl 1,3,4‐thiadiazole‐2‐amine was used as the synthon of various Schiff bases, which were prepared by reacting the amine with benzaldehyde substituents and then reacting with chloroacetic acid to form oxazolidin‐5‐one five‐membered rings. This compound has good biological activity, and physical and spectroscopic methods confirmed the structure of the prepared compound. Examples include infrared spectroscopy, proton nuclear magnetic resonance spectroscopy ( 1 H‐NMR), mass spectrometry, melting point and purity determination, and tracking of reaction progress via thin layer chromatography (TLC). Gram‐negative Escherichia coli (Gram‐ve) and Gram‐positive Staphylococcus aureus (Gram+ve), two bacterial isolates renowned for their antibiotic resistance, were used to assess the bioavailability of the produced compounds (HA6‐HA15), and the results were compared with the antibiotic amoxicillin as a control. The level of selectivity was excellent. A helium‐neon laser (visible laser) was used to measure the laser activity of several of the produced compounds (HA7, HA10, HA11, HA13, and HA15). Each compound was exposed to radiation for four durations (15, 30, 45, and 60 s). Using MOE (2015) software, the molecular binding of HA9 and H15, two of the produced compounds, to Escherichia coli was investigated.