Yield Stability and Antioxidant Response of Wheat Under Multi-Environment Conditions: Insights from AMMI and GGE Biplot Analyses

The identification of wheat genotypes with balanced grain yield, stability, and antioxidant response to abiotic stress is crucial for creating adaptable varieties in changing climates. The research was conducted as a multi-environment trial (MET) across six environments (three localities over two seasons), and included 15 wheat genotypes (bread wheat lines, standard varieties and two spelt wheat lines). This study integrates AMMI and GGE biplot analyses with antioxidant profiling across growth stages to identify genotypes combining yield stability and stress tolerance. Lines KG-4/1, KG-11/1 and the variety Pobeda were identified as high-yielding and stable genotypes, with KG-11/1 ranked as an “ideal genotype” and KG-4/1 achieving the highest grain yield on MET level (6.48 t ha–1). Specific adaptation of genotypes KG-40/1 and Renesansa indicates their potential for targeted breeding in particular environments. Antioxidant parameters (TPC and DPPH• scavenging activity) at early growth stages were positively correlated with grain yield, indicating that they can serve as suitable biochemical markers for selecting stress-tolerant, high-yielding wheat genotypes. This comprehensive approach, which includes the analysis of grain yield stability/adaptability and antioxidant parameters, is adequate for the evaluation of promising lines that may be suitable genetic resources in climate-resilient wheat breeding programs.