Molecular analyses of Cellulosimicrobium cellulans Mosul isolated from crown galls of Olea europaea

Olea europaea L. is farmed extensively for its oil in various parts of the world. Olives are abundant in bioactives and beneficial minerals that may have therapeutic and medicinal applications. Cellulosimicrobium cellulans belongs to the family Promicromonosporaceae and is classified as a Gram-positive bacillus. Since there is not much research on the topic, the goal of the current study was to analyze the whole genome sequence of this bacterium. The results showed that 99.8% of the raw values agreed well with the reference strain CP017660, with a GC content of 74.5% and a pairwise similarity of 98.1%. The de novo assembly analysis revealed that 3,250 contigs totaling 5,272,102 lengths were present, derived from 20% of the raw reads that were supplied. However, the findings showed that C. cellulans had 1772 encoding genes for proteins without functions and 2175 expressing genes for particular proteins with particular functions. The genome annotation result showed 41 completely characterized annotated features, including alanyl-tRNA synthetase, the aerotolerance protein BatB, argininosuccinate synthase, 3-hydroxyisobutyryl-CoA hydrolase, and others. The phylogenetic analysis based on the genomic DNA showed that the C. cellulans Mosul strain grouped in a clade that included closely associated strains, such as CP017660, CP041694, CP020857, CP072387, and CP017660 strains. Moreover, the heatmap matrix outcomes demonstrated a substantial relationship among C. cellulans and CP041694, CP072387, CP017660, CP020857, CP101475, and CP053419 strains. Lastly, the subsystem feature results showed that 134 genes encoding enzymes related to vitamins, pigments, cofactors, and prosthetic groups were present, along with 34 genes linked to defense, virulence, and disease.