Kolon Kanserinde Notch Yolağına Duyarlı Gen Ekspresyonlarının Araştırılması

Colorectal cancer (CRC) is a leading cause of cancer-related mortality, largely due to therapy resistance and metastasis. This study explored the role of Notch signaling in CRC by comparing the expression of pathway-associated genes between healthy CCD841-con cells and HCT116 colorectal cancer cells.Semi-quantitative PCR analysis revealed significant upregulation of GATA3 (4-fold), HES (5-fold), and HEY (3-fold) in HCT116 cells, while PTCRA expression decreased 4-fold (p < 0.005, normalized to GAPDH). These results suggest that Notch signaling contributes to tumor progression in CRC. The elevated expression of HES and HEY may be linked to epithelial-mesenchymal transition (EMT) and cancer stem cell-like traits.Overall, this study highlights the potential of targeting the Notch pathway in CRC therapy, offering insights into its role in tumor development and resistance mechanisms.