In Situ Development of Quercetin-Enhanced Layered Double Hydroxides for Targeted Osteosarcoma Therapy
In this study, we investigated the structural, thermal, and cytotoxic properties of Mg-Al layered double hydroxides (LDHs) intercalated with quercetin. Fourier transform infrared (FTIR) and Raman spectroscopy confirmed the successful incorporation of quercetin into the LDH structure, while X-ray diffraction (XRD) showed an increased interlayer spacing due to quercetin intercalation. Thermogravimetric analysis (TGA) demonstrated greater thermal degradation in quercetin-intercalated LDHs compared to nitrate-intercalated LDHs, suggesting a higher quercetin content in the material. Atomic force microscopy (AFM) indicated morphological changes with quercetin, particularly larger surface clusters at room temperature. Cytotoxicity assays revealed that quercetin-intercalated LDHs exhibited a dose- and time-dependent reduction in cell viability, which was more pronounced at higher concentrations and longer exposure times. Quercetin alone had a stronger cytotoxic effect on NIH/3T3 cells compared with Saos-2 cells. Additionally, reactive oxygen species (ROS) assays showed distinct effects of quercetin on both cell lines. These findings highlight the potential of quercetin-LDH hybrids for biomedical applications, particularly for targeted drug delivery.
Authors
- Konstantinos I. Tsamis (ORCID: https://orcid.org/0000-0002-7032-1630)
- Fotios K. Katsaros (ORCID: https://orcid.org/0000-0003-2554-9799)
- Λάμπρος Λάκκας (ORCID: https://orcid.org/0000-0001-9401-3125)
- Mohammed Subrati (ORCID: https://orcid.org/0000-0002-9349-7073)
- Konstantinos Spyrou (ORCID: https://orcid.org/0000-0002-2032-8439)
- Yannis V. Simos (ORCID: https://orcid.org/0000-0003-1764-8906)
- Dimitrios Gournis (ORCID: https://orcid.org/0000-0003-4256-8190)
- Panagiota Zygouri (ORCID: https://orcid.org/0000-0002-7137-6633)
- Dimitrios Peschos (ORCID: https://orcid.org/0000-0002-5490-0755)
- Antrea-Maria Athinodorou (ORCID: https://orcid.org/0000-0002-5060-4588)
- Vasileios Ragos (ORCID: https://orcid.org/0000-0002-0523-0476)
- Zili Sideratou (ORCID: https://orcid.org/0000-0002-9314-2520)
- Eirini Papanikolaou (ORCID: https://orcid.org/0000-0002-1554-2430)
- Marina Lianou
- Angela S. Kaloudi (ORCID: https://orcid.org/0009-0006-4052-0002)
- Grigorios Tsiodoulos
Institutions
- University of Ioannina (GR)
- National Centre of Scientific Research "Demokritos" (GR)
- CIBBIM-Nanomedicine (ES)
- National Medical Research Center of Dentistry and Maxillofacial Surgery (RU)
- Technical University of Crete (GR)
- RMIT University (AU)
- Foundation for Research and Technology Hellas (GR)
Publication Details
- Journal
- ACS Biomaterials Science & Engineering
- Published
- 2025-11-21
- DOI
- https://doi.org/10.1021/acsbiomaterials.5c01290
- Primary Topic
- Layered Double Hydroxides Synthesis and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00