Red-shifted excitation enhances the sensitivity of red genetically encoded Ca2+ indicator and enables crosstalk-free two-photon holographic optophysiology
Red-shifted excitation was essential for detecting the small <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"> <mml:mrow><mml:msup><mml:mi>Ca</mml:mi> <mml:mrow><mml:mn>2</mml:mn> <mml:mo>+</mml:mo></mml:mrow> </mml:msup> </mml:mrow> </mml:math> elevation following optogenetic stimulation. This optimized condition improves the sensitivity of red GECIs and enables a more robust 2P optophysiology free from crosstalk.
Authors
- Hisao Tsukamoto (ORCID: https://orcid.org/0000-0003-4138-7398)
- Hiroaki Wake (ORCID: https://orcid.org/0000-0002-8543-4590)
- Miyuki Nishi (ORCID: https://orcid.org/0000-0001-6055-7845)
- Manoj Kumar (ORCID: https://orcid.org/0000-0003-2870-0677)
- Naru Yoneda (ORCID: https://orcid.org/0000-0001-9964-5420)
- Mitsuhiro Morita (ORCID: https://orcid.org/0000-0002-9891-0242)
- Osamu Matoba (ORCID: https://orcid.org/0000-0001-9767-0059)
- P Gore
Institutions
- Kobe Design University (JP)
- Imagineering (Japan) (JP)
- Nagoya University (JP)
- Kobe University (JP)
Publication Details
- Journal
- Journal of Biomedical Optics
- Published
- 2025-11-22
- DOI
- https://doi.org/10.1117/1.jbo.30.11.116003
- Primary Topic
- Photoreceptor and optogenetics research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Australian National University
- Japan Society for the Promotion of Science
- Core Research for Evolutional Science and Technology