A robust method for primary cerebellar culture and genetic manipulation of Purkinje cells from postnatal mice
This protocol provides an efficient framework for the culture, development, and genetic manipulation of Purkinje cells, facilitating their application in molecular, cellular, and functional studies of cerebellar development and disease models.
Authors
- Friederike Stephani
- Edwin Mientjes (ORCID: https://orcid.org/0000-0002-6700-9259)
- Catarina Osório
- Martijn Schonewille (ORCID: https://orcid.org/0000-0002-2675-1393)
- Heiling Lu (ORCID: https://orcid.org/0000-0001-6791-1771)
- Myrna Aulia
Institutions
- Erasmus MC (NL)
Publication Details
- Journal
- Journal of Neuroscience Methods
- Published
- 2025-11-22
- DOI
- https://doi.org/10.1016/j.jneumeth.2025.110627
- Citations
- 1
- Primary Topic
- Neurogenesis and neuroplasticity mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 1.32