One-step generation of sh2isu1 sweet maize via CRISPR/Cas9 cytosine base editor (CBE)
1. This study represents the first report of the precise generation of sh2isu1 compound sweet maize germplasm utilizing the CRISPR/Cas9 cytosine base editing system in the maternal inbred line of a dominant Chinese maize variety. 2. By employing gene editing technology, we successfully and efficiently generated high-quality sweet maize target germplasm with precision and isolated the homozygous sh2isu1 mutant devoid of transgenic elements within a timeframe of 1 to 2 years.
Authors
- Jiuran Zhao (ORCID: https://orcid.org/0000-0002-5538-7236)
- Ziheng Song (ORCID: https://orcid.org/0009-0007-7015-8291)
- Yao Wang (ORCID: https://orcid.org/0000-0001-6164-8167)
- Ya Liu (ORCID: https://orcid.org/0000-0001-8988-3644)
- Yue Fu (ORCID: https://orcid.org/0000-0002-8410-678X)
- Mengyuan Liu (ORCID: https://orcid.org/0000-0002-6332-8316)
- Xiaoxu Li (ORCID: https://orcid.org/0000-0001-8833-9401)
- P.-W. Wang
- Lu Zhang (ORCID: https://orcid.org/0009-0005-9929-5420)
- Ronghuan Wang
Institutions
- Beijing Academy of Agricultural and Forestry Sciences (CN)
Publication Details
- Journal
- Journal of Integrative Agriculture
- Published
- 2025-11-21
- DOI
- https://doi.org/10.1016/j.jia.2025.11.031
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00