Peptide nucleic acid (PNA) clamps enhance root microbiome profiling in wheat and maize
In conclusion, the introduction of universal PNA clamps during PCR assays significantly reduced amplification of host contamination and enhanced the detection of low-abundance microbiome and the depth of microbial profiling in both maize and wheat root tissues, with effects being concentration- and crop-specific.
Authors
- Dânia Vieira Branco Ozório
- Daniel P. Schachtman (ORCID: https://orcid.org/0000-0003-1807-4369)
- Cody F. Creech (ORCID: https://orcid.org/0000-0002-5334-4814)
- Hussnain Mukhtar (ORCID: https://orcid.org/0000-0002-9312-1594)
- Amanda C. Easterly (ORCID: https://orcid.org/0000-0002-1815-5049)
- Kaitlin B. Bachle
Institutions
- University of Nebraska–Lincoln (US)
Publication Details
- Journal
- Environmental Microbiome
- Published
- 2025-11-21
- DOI
- https://doi.org/10.1186/s40793-025-00808-0
- Primary Topic
- Biosensors and Analytical Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.00