Validated spectrophotometric method for the determination of cefepime in pure and pharmaceutical foumulation

A simple, precise, rapid sensitive and accurate Spectrophotometric method has been developed for the estimation of Cefepime as pure form and in its formulation. This method is based on reductive complex reaction of Cefepime with 2,2’-Bipyridine at pH - 4 in the presence of Ferric chloride to form orange red colored product which is extractable at 520 nm. Beer’s law is obeyed in the concentration range 5-30 µg.ml -1 . The product obeyed Beer’s law with molar absorptivity of 1.0534×10 4 l. Mol -1 cm -1 . Sandell’s sensitivity 0.04561 µg.cm 2 and RSD% was found to be 0.4254% and recovery of 99.77%. The method was completely validated and proven to be rugged. The interferences of the ingredients and excipients were not observed. The repeatability and the performance of the proved method were established by point and internal hypothesis and through recovery studies.