In vitro study on the bacteriostatic effect of Amoxicillin-loaded Nano-hydroxyapatite and its capability in occluding dentinal tubules

This study explores the potential of Carboxymethy Cellulose/Amoxicillin/Nano-Hydroxyapatite suspensions (CMC/AMX/nHA) for the treatment of dentin hypersensitivity, particularly in the context of periodontitis treatment. The CMC/AMX/nHA suspension was compared with the commonly used clinical desensitizing agent Bifluorid12 and the broad-spectrum antibiotic Minocycline Hydrochloride. Microscopic observation and antimicrobial experiments were conducted to quantitatively and qualitatively assess the ability of CMC/AMX/nHA to seal dentinal tubules and its antimicrobial properties. Additionally, a resin bonding experiment was performed to evaluate the effect of CMC/AMX/nHA on dentin bonding. The research results indicate that CMC/AMX/nHA demonstrates good biocompatibility, with excellent drug delivery capabilities, bacterial growth inhibition, and no interference with subsequent repair treatments during desensitization therapy. These findings suggest that CMC/AMX/nHA has high translational potential and may be effectively applied in clinical oral medicine.