Characterization of Terpenoids in Aromatic Plants Using Raman Spectroscopy and Gas Chromatography–Mass Spectrometry (GC–MS)

The study characterized the essential oils of eight aromatic medicinal plants (<i>Tagetes filifolia</i>, <i>Aloysia citrodora</i>, <i>Cymbopogon citratus</i>, <i>Eucalyptus globulus</i>, <i>Chamaemelum nobile</i>, <i>Piper aduncum</i>, <i>Minthostachys mollis</i>, and <i>Rosmarinus officinalis</i>) using Raman spectroscopy and gas chromatography-mass spectrometry (GC-MS). Raman spectra allowed the identification of bands associated with C-H, C=C, C-O, and C=O bonds, evidencing the presence of monoterpenes, sesquiterpenes, and oxygenated compounds. GC-MS analysis confirmed these results, detecting 224 compounds, predominantly terpenoids. Among the major compounds, cis,cis-nepetalactone (30.16%), β-caryophyllene (up to 18.26%), citronellol (10.92%), citral, and linalool were found. The combination of both techniques made it possible to relate the chemical composition to the molecular structure. This showed that the differences between species are mainly due to the proportion of oxygenated compounds (citral, linalool, geraniol) compared with aromatic hydrocarbons (β-caryophyllene, D-limonene, β-pinene). Additionally, the presence of cis,cis-nepetalactone in M. mollis was reported for the first time, representing a significant chemical finding.