Elevating Flow Cytometric Analysis: A Comparative Study of Traditional and Image-Enhanced Methods Using the Invitrogen™ Attune™ CytPix™ Flow Cytometer 3065

Abstract Description There are demonstrative advantages of image-enhanced flow cytometry. These advantages are further strengthened when utilizing machine learning to automate image analysis and evaluate morphological parameters along with antibody based immunophenotypic panels. A series of experiments were conducted using the Invitrogen™ Attune™ CytPix™ Flow Cytometer to compare traditional flow cytometry with image-enhanced flow cytometry, focusing on aspects such as viability staining, aggregate exclusion, no-wash no-lyse protocols, and cell stimulations. The integration of the imaging capabilities within the Attune CytPix Flow Cytometer allows both marker expression and cellular morphology to be used to facilitate even higher dimensional flow cytometric analysis. The resulting flow cytometry data identified distinct cell populations within biological samples, which were more precisely characterized using imaging parameters, thereby improving the accuracy of phenotypic analysis. These findings underscore the effectiveness of the Attune CytPix Flow Cytometer for image-enhanced flow cytometry in multiparametric immune cell phenotyping, providing valuable insights for immunological research and clinical diagnostics. For Research Use Only. Not for use in diagnostic procedures. Topic Categories Technological Innovations in Immunology (TECH)