Investigating and understanding the key transporters (membrane protein) responsible for clearing the released neurochemical from the synaptic cleft in mesenteric lymph node 2249

Abstract Description Neuroimmune communication is a key component in regulating the body’s response to physiological challenges and maintaining homeostasis while facilitating immune stress response. Within the gastrointestinal tract, the mesenteric lymph nodes play a key role in governing the immune response, and this regulation involves communication between immune cells and neural components. Previously, our lab has measured signaling of catecholamine (CA) from sympathetic neurons from naive murine lymph nodes with Fast-Scan Cyclic Voltammetry at carbon-fiber micro-electrodes. Sympathetic neurons are thought to release norepinephrine (NE) to modulate cytokine signaling during inflammation and are reuptake by different transporter. Therefore, measurement of these released neurochemicals and clearance mechanism in the lymph node are critical for understanding immunity. CA transporters are know to be on the plasma membrane of presynaptic neurons and on immune cells to aid in NE clearance from the synaptic cleft. We used different pharmacological application for specific transport inhibition to study the major transporter involved and found out that there are diverse group of transporters (NE, Dopamine, Serotonin, Organic cation 1/3) into play. With western blot and flow cytometry we showed transporter present on each cell type. This work provide the 1st time detection of CA on sub-second time scale which will provide a insight into the mechanism of neurochemical regulated immunity and beyond. Topic Categories Neuroimmunology (NEUR)