Transcriptomic subtypes and immune infiltration in high-grade serous ovarian cancer 4644

Abstract Description High-grade serous ovarian cancer (HGSOC) is a highly aggressive malignancy with a heterogeneous molecular landscape. This study investigates the interplay between mRNA subtypes and the tumor immune microenvironment (TIME) in HGSOC. We analyzed transcriptomic data to characterize four mRNA subtypes: immunoreactive, differentiated, proliferative, and mesenchymal. Using DESeq2 and WGCNA, we identified gene modules associated with clinical traits and immune cell infiltration. The immunoreactive subtype exhibited significant enrichment of M1/M2 macrophages, Th1 cells, and B cells, along with LAIR-1, FCRL5, and IRF4 expression. The mesenchymal subtype was associated with fibroblasts, while the proliferative subtype showed strong ovarian stromal cell involvement. Our findings reveal distinct immune landscapes associated with each mRNA subtype, suggesting potential therapeutic targets and prognostic markers for HGSOC. Funding Sources NIH/NCI U54CA118638; Chan Zuckerberg Initiative CZIF2002-007045 Topic Categories Tumor Immunology: Cellular Responses and Tumor Microevironment (TIME)