Automate tissue processing with STEMprep™ for versatile sample preparation 3334

Abstract Description Tissue environments are specialized to support distinct functions. Analyzing cell interactions and gene expression within tissues is essential for advancing tissue research and understanding disease mechanisms. Tissue samples must first be processed with protocols that preserve cell viability, yield and functionality. However, achieving an optimal balance between enzymatic and mechanical dissociation for tissues can be time-consuming and technically demanding. To address this, we developed the STEMprep™ Tissue Dissociation system to efficiently generate single cells from tissue samples. This system features an instrument with integrated temperature control and tissue-specific programs, specialized sample tubes, and kits for enzymatic digestion. Our results demonstrate high cell viability and yield for mouse spleen (95.3 ± 2.7%, 1.2E8 ± 3.2E7 cells/tissue), brain (89.2 ± 4.7%, 2.6E6 ± 5.6E5 cells/tissue), lung (90.6 ± 2.9%, 1.5E7 ± 4.0E6 cells/tissue), liver (90.0 ± 5.1%, 6.1E7 ± 6.7E7 cells/tissue), and CT26 tumors (82.9 ± 6.1%, 2.6E4 ± 1.3E4 cells/mg tissue). STEMprep™-processed samples are compatible with EasySep™ cell isolation, and functional in cell-specific downstream assays. High-quality RNA can also be extracted directly using a homogenization protocol, enabling gene analysis workflows. The STEMprep™ Tissue Dissociation system streamlines tissue processing compared to manual procedures, enhancing consistency, throughput, and efficiency to accelerate tissue research. Topic Categories Technological Innovations in Immunology (TECH)