Impact of sleep deprivation on monocyte subclasses and inflammation: insights from a cross-sectional and controlled study 4119

Abstract Description The relationship between sleep deprivation, obesity, and systemic inflammation is a critical area of investigation due to its significant impact on health. While it is established that poor sleep adversely affects obesity and metabolic syndromes, the specific mechanisms, particularly subclinical inflammation independent of obesity, remain unclear. This study investigates how sleep quality influences monocyte subclass distribution and its association with systemic inflammation across a spectrum of BMI categories. In our cohort study, 237 healthy participants were categorized by body mass index. Participants’ dietary intake, physical activity, and sleep patterns were objectively tracked. The data showed that obese individuals had significantly lower sleep quality and higher chronic low-grade inflammation. Non-Classical Monocytes (NCM) increased significantly in obesity, correlating with reduced sleep quality and elevated pro-inflammatory cytokines. Although BMI emerged as a significant factor in driving inflammation, mediation analyses further defined that sleep disruption independently contributes to inflammation, regardless of obesity status. Controlled sleep deprivation experiments confirmed these findings, demonstrating reversible increases in NCM expression. This study highlights the importance of sleep quality in regulating immune responses and inflammation in obesity, suggesting that improving sleep quality could reduce inflammation and improve health outcomes. Funding Sources This work was supported by grants (RH HM-2019-019) from Kuwait Foundation for the Advancement of Sciences (KFAS). Topic Categories Translational and Interventional Immunology (TI)