Immunological impacts of exposure to microplastic water contaminants during early development in Xenopus 2357

Abstract Description The amphibian Xenopus immune system shares fundamental similarities with that of humans, making it a sensitive and reliable model for studying the impact of environmental toxicants on the developing immune system. Both species possess innate and adaptive immune components. Important signaling pathways and immunoregulatory cytokines are conserved between Xenopus and humans. Thus, the Xenopus model provides valuable insights into fundamental immunological processes and can help elucidate the impacts of environmental pollutants on human immune defenses against pathogens. Utilizing Xenopus as a relevant model for assessing human health implications, our research demonstrates that upon exposure to environmentally representative mildly weathered post-consumer polyethylene terephthalate microplastics (PET MPs), there is a rapid accumulation observed within tadpole intestine, liver, and kidneys, persisting over a week. This accumulation leads to compromised antiviral immunity and diminished resistance against viral infections. Preliminary data also suggest that PET MPs affect MHC-II expression and macrophage function. Ongoing investigations are delving into potential long-term immune deficits resulting from developmental exposure to PET MPs post-metamorphosis in adult frogs. These findings carry substantial significance, raising developmental immunotoxicity (DIT) concerns not only for aquatic vertebrates but also for human health. Funding Sources Institute of Allergy and Infectious Diseases (NIH/NIAID), as well as a Research Program Project P01ES035526 from the National Institute of Environmental Health Sciences,and a Pilot Project Grant from the Rochester Environmental Health Sciences Center (P30-ES01247). Topic Categories Veterinary and Comparative Immunology (VET)