Characterization of atopic dermatitis patients is defined by immune cell infiltrate and its association with etiological factors 4585

Abstract Description Skin diseases affect the quality of life of people who suffer from it. Atopic dermatitis (AD) and psoriasis (PS) are part of the most common causes of dermatological consultation in Mexico. Diagnosing AD can be challenging due to its multifactorial etiology and similarity of lesions from other dermatoses. Therefore, characterizing AD in an integral manner could lead to better understanding of the disease by creating a cellular and molecular hallmark for AD. In this study, 39 AD patients, 25 PS patients and 15 healthy controls were analyzed by immunofluorescence to identify the main markers that differentiate AD from PS. Results showed higher MFI for filaggrin (FL) and and involucrin (IV) proteins for PS patients than AD patients. While tight junctions, claudin 1 and 4 had no MFI differences in the 3 groups, AD patients showed major presence of occludin in keratinocytes and dermis cell infiltrate. Subsets of T CD4+ cells were quantified, obtaining a larger proportion of Th2, Th17, Th22 and Treg infiltrate in AD showing a greater activity of T CD4+ in this disease. Innervation, Substance P and CGRP neuropeptides presence were evaluated, finding significant increase for these makers in AD. Finally, a PCA was made to identify the main markers of AD, were the infiltrate of CD4+ T cells (Th2, Th22, Th17), along with the high presence of nerve endings and cells with the neuropeptides CGRP, seems to play an important role in the differentiation of atopic dermatitis samples. Funding Sources Conahcyt CVU: 1225279 SEDENA Topic Categories Translational and Interventional Immunology (TI)