Expansive immunophenotyping of natural killer cell populations with the InvitrogenTM AttuneTM XenithTM flow cytometer 4261

Abstract Description As the field of flow cytometry expands our knowledge of the immune system and increased panel complexity has put a greater demand on instrumentation. Spectral unmixing has advanced panel size and diversity, enabling the study of novel cell populations. Using the Invitrogen Attune Xenith flow cytometer, we have developed a 31-color panel to investigate NK cell lineages and complimentary T cell populations. With the use of acoustic focusing technology, we demonstrate the ability to separate rare cell types, such as invariant NKT cells (iNKTs) and innate lymphoid cell (ILC) subsets at flow rates up to 500 μl/min. Additionally, we investigate activation and inhibition markers on various NK cell populations both with and without cytokine stimulation. Using the Xenith flow cytometer we are able to incorporate near-infrared excited dyes (such as LIVE/DEAD™ Fixable Near IR 876 viability dye) to expand scope of the panel while reducing spectral unmixing burden. This expanded detection array allows for future panel expansion and flexibility to suit the needs of further research efforts in the NK and CAR-NK space. The spectral capabilities of the Xenith flow cytometer, coupled with the clog-resistant acoustic focusing allow users to run samples at faster speeds, reducing time to results even for deep immunophenotyping of rare cell populations. Topic Categories Technological Innovations in Immunology (TECH)