Real time analysis of neurochemical signaling from regulatory T-Cells (Tregs) with fast scan cyclic voltammetry 3718

Abstract Description Neurochemicals play a pivotal role in dictating communication within the body. Recent studies have demonstrated that neurochemical signaling modulates communication between immune cells and neurons. Neurodegenerative diseases are hypothesized to be influenced by these neuron-immune interactions. Understanding the primary neurochemicals released from immune cells can help understand the dynamics of neuro-immune signaling and molecular mechanism of neuroinflammation. While research has explored neuro-immune interactions, studies on the rapid release of neurochemicals within immune cells are limited. Here, we have discovered the rapid release of neurochemicals from Tregs, a specialized subset of T lymphocytes that play a crucial role in preventing autoimmunity. Literature suggests that neurochemical’s effect on suppressing Treg function, which is linked to various neurological disorders, highlights the potential therapeutic value of neurochemical production and release by Tregs for treating these diseases. We have used Fast Scan Cyclic Voltammetry (FSCV); a powerful electrochemical technique with excellent spatial and temporal resolution to detect neurochemicals in this sub-second timescale. Our findings so far demonstrate that Tregs produce different types of neurochemicals on the same timescale as neurons. We also performed flow cytometry analysis to determine which transporters present on Tregs which are important for neurochemical reuptake and release mechanism. Funding Sources NIH R21AG086735 Topic Categories Immune Response Regulation: Cellular Mechanisms (IRC)