Immunomodulatory effects of a spermine-binding peptide vaccine in a 3D MyC-CaP prostate tumor model 2930

Abstract Description Objectives 1) Evaluate the therapeutic potential of the spermine-binding peptide vaccine (p25 99-118) in modulating tumor progression in a 3D MyC-CaP prostate tumor model. 2) Investigate the immune response induced by p25 99-118, focusing on CD4+ and CD8+ T cell infiltration. Methods FVB mice were immunized intraperitoneally with p25 99-118 emulsified in complete Freund’s adjuvant. Post-immunization, splenocytes were harvested and co-cultured with MyC-CaP spheroids in 3D scaffolding matrix plates. Dose-dependent effects were evaluated by adding increasing concentrations of p25 99-118, with OVA 323–339 serving as a negative control. Fixed spheroids were stained with fluorophore-conjugated anti-CD4 and anti-CD8 for confocal microscopy and then T cell infiltration quantified. Results The p25 99-118 vaccine significantly inhibited spheroid growth. Treated spheroids showed robust CD4+ and CD8+ T cell infiltration, while controls exposed to OVA 323–339 exhibited minimal infiltration, confirming antigen specificity. Conclusions The p25 99-118 vaccine induces a T cell-mediated immune response, significantly reducing tumor growth in a 3D model and highlighting its therapeutic potential in prostate cancer. Topic Categories Vaccines and Immunotherapy (VAC)