B cell subsets have different capacities for phagocytosis and subsequent presentation of antigen to cognate T cells 2920

Abstract Description B cells have been shown to be phagocytic under some circumstances. However, the phagocytic capacity of different B cell subsets and how this is linked to antigen presentation or other functions has not been characterized. To address this, we developed 2 µm phagocytic antigen conjugated bead targets that target phagocytic pathways including the BCR, scavenger, Fc, and complement pathways to study potential pathways by which B cells phagocytose both cognate and non-cognate antigen. We found that while follicular B2, marginal zone, and B1 B cells are highly phagocytic of BCR-engaging targets through their BCR, only peritoneal cavity B1 cells could uptake non-cognate antigen-coated beads or bacteria. Despite this, B1 cells were not effective at presenting antigen to activate cognate T cells or at killing phagocytosed bacteria. Finally, analysis of scRNA-seq data revealed that these differences in phagocytic capacity could not be explained by differential expression of relevant phagocytic receptors, implying that there is likely some form of regulation in place preventing non-cognate antigen uptake by follicular B2 cells. Our work will help contribute to a better understanding of non-classical antigen uptake mechanisms employed by B cells and their relevance to inflammation. Topic Categories Antigen and Dendritic Cell Processing, Presentation, and Biology (AGDC)