Streamlined spatial analysis of the tumor microenvironment and associated disease states using multiplex immunohistochemistry 9329

Abstract Description Spatial studies of the tumor microenvironment are crucial for understanding the complex interactions between different cell types and how these interactions contribute to disease progression. This poster presents a streamlined approach to spatial analysis of the tumor microenvironment in brain tissues, focusing on astrocytoma and oligodendroglioma. Additionally, the methodology was applied to Parkinson’s Disease versus healthy brain tissue to observe disease-specific alterations. We employed primary antibody conjugates to perform immunohistochemistry (IHC) on human tissue samples, utilizing an 8-plex panel of markers. The study incorporated IHC-validated antibody conjugates supplemented by an antibody labeling kit for disease-specific markers, facilitating high spatial resolution imaging without the need for lengthy workflows commonly associated with secondary amplification or cyclic stripping methods. Our findings demonstrate a significant increase in astrocytes and oligodendrocytes in the tumor tissue, as revealed by the increased presence of GFAP and Olig2, respectively, compared to healthy controls. The use of the EVOS S1000 imaging system made image capture and analysis remarkably straightforward, further simplifying the workflow. This approach provides clear advantages over traditional methods, highlighting its potential for broad application in immunological and neuropathological studies. For Research Use Only. Not for use in diagnostic procedures. Funding Sources Thermo Fisher Scientific Topic Categories Tumor Immunology: Cellular Responses and Tumor Microevironment (TIME)