Immune response measurement of antigen-specific B cells by Elispot 2293

Abstract Description The plasma and memory B-cell ELISPOT is an extremely sensitive assay based on the detection of antigen specific secreted antibodies from cryopreserved or fresh PBMCs. The B cell response encompasses four immunoglobulin (Ig) classes (IgG/M/A/E) and four IgG subclasses (IgG 1-4), each contributing to fundamentally different effector functions. For clinical studies assessment of humoral immunity to infectious agents and hence to antigen-specific B cell responses is most often confined to the antibodies present in serum, as evaluated by ELISA or NAb assays. It neglects monitoring memory B cells, which could mount a faster and more efficient antibody response after antigen re-encounter. In immunotherapy evaluations, ELISA measurements of total IgG are usually performed without taken into consideration the detection of the appropriate Ig class or IgG subclasses, which are critical for successful host defense and avoidance of immunopathology. Our multiplexed ELISPOT/FluoroSpot (collectively ImmunoSpot®) assay platform is ideally suited for antigen-specific B-cell enumeration, since it provides a single-cell resolution for counting individual antibody-secreting cells (ASC), might this be from plasma or memory B cells. This information cannot be provided by ELISA assays. In summary, we will show successful measurements of antigen specific B cells in clinical settings after infection or vaccination, facilitating high-throughput immune-monitoring efforts of large donor cohorts. Funding Sources Supported by Cellular Technology Limited Topic Categories Technological Innovations in Immunology (TECH)