Characterization of cellular diversity and immune landscape in Mouse Glioblastoma using whole slide imaging modes of high-plex Imaging Mass Cytometry 2619

Abstract Description Mouse models are pivotal for translational research, enabling the detection of therapeutic-induced immune modifications within the tumor microenvironment (TME). We utilized a 40-marker panel targeting tumor and immune markers to investigate the spatial protein composition of the whole mouse embryo, mouse brain and mouse glioblastoma (GBM) tumor sections. Imaging Mass Cytometry™ (IMC™) technology was employed to capture the expression of 40 markers simultaneously while preserving tissue integrity and enabling a flexible workflow without autofluorescence artifacts. Data analysis revealed distinct spatial patterns of tumor and immune markers across the GBM, identifying key features such as necrotic cores, regions of high immune infiltration, proliferating tumor cells and diverse populations of infiltrating immune cells. The wide detection range of expression provided by IMC technology enabled visualization and analysis of clusters exhibiting varying levels of key GBM markers, suggesting a probable dual origin of the GBM, coexistence of pro- and anti-tumorigenic immune responses and overall extreme heterogeneity that can only be visualized with high-plex antibody panels. This approach demonstrates the capability of IMC technology to reveal the complex cellular and structural spatial landscape of the TME in GBM, providing valuable insights into tumor biology and potential therapeutic targets for patient stratification. For Research Use Only. Not for use in diagnostic procedures. Topic Categories Tumor Immunology: Cellular Responses and Tumor Microevironment (TIME)