Extreme environmental tolerance and space survivability of the moss, Physcomitrium patens

Climate change highlights the importance of understanding life's survival limits for addressing global challenges and supporting future human habitation beyond Earth. Plants, as photosynthetic organisms, play a vital role in sustaining life. Bryophytes, such as mosses, show notable extremotolerance, but despite studies on environmental responses in the model moss <i>Physcomitrium patens</i>, its survival under extreme conditions in space remains unclear. We tested protonemata, brood cells, and spores encased in sporangium under simulated space environments, identifying spores as the most resilient, and subsequently exposed them to the space environment outside the International Space Station. After 9 months in space, over 80% of the spores survived, retaining their ability to germinate. These results demonstrate the remarkable resilience of <i>P. patens</i> spores in space and reveal the potential of terrestrial plants to endure extreme environments, providing insight into bryophyte adaptation and offering a foundation for future applications in space exploration and extraterrestrial habitats.