Protocol for a mouse tubuloid model of myoglobinuric acute kidney injury
Myoglobinuric acute kidney injury (AKI) occurs as a result of rhabdomyolysis. Here, we present a protocol based on mouse primary renal tubular epithelial organoids, or "tubuloids", to model myoglobinuric AKI in vitro. We describe how to establish and maintain the culture of mouse tubuloids and how to trigger myoglobin-induced injury. Moreover, we provide instructions on how to quantify tubuloid damage. For complete details on the use and execution of this protocol, please refer to Zhou et al.<sup>1</sup>.
Authors
- Marc Torres Pereiro (ORCID: https://orcid.org/0000-0003-3359-7715)
- Thomas Worzfeld (ORCID: https://orcid.org/0000-0003-3908-403X)
- Luping Zhou (ORCID: https://orcid.org/0000-0003-1065-6604)
Institutions
- Philipps University of Marburg (DE)
- Southwest Medical University (CN)
- Affiliated Hospital of Southwest Medical University (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-11-20
- DOI
- https://doi.org/10.1016/j.xpro.2025.104205
- Primary Topic
- Muscle and Compartmental Disorders
- Type
- article
- Field-Weighted Citation Impact
- 0.00