A comparative study of DNA- and RNA-metagenomic next-generation sequencing for pathogen detection in lower respiratory tract infections
DNA-mNGS and RNA-mNGS exhibited low overall consistency. However, RNA-mNGS showed superior precision in identifying causative pathogens in LRTI and additional capacity for RNA virus detections, while DNA-mNGS possessed essential sensitivity for low abundance pathogens.
Authors
- Li Weng (ORCID: https://orcid.org/0000-0003-0403-0752)
- Tianyuan Zhu (ORCID: https://orcid.org/0000-0001-5007-5762)
- Bin Du
- Dong Zhang
- Jiayu Guo (ORCID: https://orcid.org/0000-0002-3616-757X)
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Peking Union Medical College Hospital (CN)
Publication Details
- Journal
- Journal of Infection
- Published
- 2025-11-20
- DOI
- https://doi.org/10.1016/j.jinf.2025.106659
- Primary Topic
- Respiratory viral infections research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Ministry of Science and Technology of the People's Republic of China
- Chinese Academy of Medical Sciences
- Peking Union Medical College Hospital